Can MDAMB-231 cell be grown in DMEM media?

More Heena Jariyal's questions See All

Sorting MCF-7 cancer stem cells ?

hello, Is there anyone who has sorted MCF-7 using CD24-/ CD44++/CD326++(epcam) markers. I got articles with CD24/44. Please share some articles with FACS profile from mcf-7 and MDAMB 231 with...

04 May 2018 2,468 0 View

Lentiviral packaging Vectors?

Hello, everyone, I need plasmid for Lentiviral formation. Is there any lab in India which is using Lentiviral packaging plasmid. psPAX2 and pMD2.G. Please let us know.

08 September 2017 8,941 0 View

LDHA ASSAY optimization?

We are doing LDHA assy for mammalian cells. we are using conc enzyme .15 to .5 u/ml, pyruvate 2mM or NADH 20microM, for the standard curve but we are getting almost same absorbance. Please...

08 September 2017 6,162 0 View

Tumorsphere assay cell count ?

I am trying to isolate cancer stem cells from MCF-7 and MDA MB231 with the help of tumorsphere assay. Is sorting necessary before tumorsphere assay cell seeding or do we sort cells after assay?...

08 September 2017 5,268 6 View

PLKO1 vectors transduction?

We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...

08 September 2017 4,961 7 View

Pyruvate kinase M2 assay?

We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...

08 September 2017 2,943 5 View

Which form of sh RNA is best for experimental purposes?

We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...

06 July 2017 10,099 3 View

Sodium phosphate buffer and potassium phosphate buffer

is there a major difference between sodium phosphate buffer and potassium phosphate buffer. can they be used alternatively?

06 July 2017 9,031 4 View

Regarding pLKO1 vectors?

Hello, I want to study knockdown of a gene in cell. we are planing to buy it from sigma I have chosen the vector with shRNA insert but I am not able to understand how to choose scramble for this...

04 May 2017 8,429 1 View

Characterization of cancer stem cells?

Hello, I have isolated cancer stem cells from MCF-7 cells. I want to characterize these cells. Is there any option available other than FACS? Can we do it with PCR or western blot for markers of CSCs?

03 April 2017 427 5 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Mamatha M Pillai

Yes, you can grow this cell line in DMEM .

Agnieszka Bojko

I am culturing them in DMEM high glucose and they are doing perfectly fine. Best regards!

Susanne Reichinnek

Hi Heena * If you like to have a standradized solution, you could use this one, too http://www.promocell.com/app/uploads/2018/01/C-28070.pdf

Good luck with the experiments!