Sodium phosphate buffer and potassium phosphate buffer

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Steingrimur Stefansson

Hi Heena, if you are doing tissue culture, then there should be no difference,

If you are planning to do SDS-PAGE, stay away from K2PO4 buffers because they precipitate with SDS..

Heena Jariyal

Hi stefansson,

So it will depend on the particular protocol. May they be interchanged?

Hi Heena, what protocols are you planning to interchange?

Actually we are planing for cytochrome c oxidase assay and HPLC for detection of ATP and NADH. HPLC assay have Potassium phosphate as mobile phase and in cyto chrome c assay they have used sodium phosphate buffer. so can we used them alternatively