Hello, everyone,
I need plasmid for Lentiviral formation. Is there any lab in India which is using Lentiviral packaging plasmid. psPAX2 and pMD2.G. Please let us know.
hello, Is there anyone who has sorted MCF-7 using CD24-/ CD44++/CD326++(epcam) markers. I got articles with CD24/44. Please share some articles with FACS profile from mcf-7 and MDAMB 231 with...
04 May 2018 2,468 0 View
We are doing LDHA assy for mammalian cells. we are using conc enzyme .15 to .5 u/ml, pyruvate 2mM or NADH 20microM, for the standard curve but we are getting almost same absorbance. Please...
08 September 2017 6,162 0 View
I am trying to isolate cancer stem cells from MCF-7 and MDA MB231 with the help of tumorsphere assay. Is sorting necessary before tumorsphere assay cell seeding or do we sort cells after assay?...
08 September 2017 5,268 6 View
We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...
08 September 2017 4,961 7 View
We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...
08 September 2017 2,943 5 View
I want to do tumorsphere assay with MDAMB231 it is usually grown in L-15 or RMI 1640 media. So, do I need to prepare cancer stem cell CSCs specific media in L-15 or can I use CSCs media prepared...
07 August 2017 8,207 3 View
We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...
06 July 2017 10,099 3 View
is there a major difference between sodium phosphate buffer and potassium phosphate buffer. can they be used alternatively?
06 July 2017 9,031 4 View
Hello, I want to study knockdown of a gene in cell. we are planing to buy it from sigma I have chosen the vector with shRNA insert but I am not able to understand how to choose scramble for this...
04 May 2017 8,429 1 View
Hello, I have isolated cancer stem cells from MCF-7 cells. I want to characterize these cells. Is there any option available other than FACS? Can we do it with PCR or western blot for markers of CSCs?
03 April 2017 427 5 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
What is the harnessing rainwater harvesting for climate change adaptation and how is India trying to ensure rainwater harvesting?
20 July 2024 6,041 2 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
What was the impact of the reform movement on India and impact of land reforms on small and marginal farmers?
09 July 2024 9,781 0 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
No explanation or detail is required to understand the question.
03 July 2024 3,958 0 View
Has India been the largest exporter of virtual water in the world and virtual water trade affect the local environment and relationship between virtual water and a water footprint?
03 July 2024 4,529 7 View