Sorting MCF-7 cancer stem cells ?

hello, Is there anyone who has sorted MCF-7 using CD24-/ CD44++/CD326++(epcam) markers. I got articles with CD24/44. Please share some articles with FACS profile from mcf-7 and MDAMB 231 with...

04 May 2018 2,260 0 View

LDHA ASSAY optimization?

08 September 2017 6,027 0 View

Tumorsphere assay cell count ?

08 September 2017 5,120 6 View

PLKO1 vectors transduction?

We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...

08 September 2017 4,824 7 View

Pyruvate kinase M2 assay?

We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...

08 September 2017 2,800 5 View

Can MDAMB-231 cell be grown in DMEM media?

I want to do tumorsphere assay with MDAMB231 it is usually grown in L-15 or RMI 1640 media. So, do I need to prepare cancer stem cell CSCs specific media in L-15 or can I use CSCs media prepared...

07 August 2017 8,079 3 View

Which form of sh RNA is best for experimental purposes?

06 July 2017 9,969 3 View

Sodium phosphate buffer and potassium phosphate buffer

is there a major difference between sodium phosphate buffer and potassium phosphate buffer. can they be used alternatively?

06 July 2017 8,757 4 View

Regarding pLKO1 vectors?

04 May 2017 8,323 1 View

Characterization of cancer stem cells?

Hello, I have isolated cancer stem cells from MCF-7 cells. I want to characterize these cells. Is there any option available other than FACS? Can we do it with PCR or western blot for markers of CSCs?

03 April 2017 293 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View