Ninhydrin Test with not so free amines?

Hi, Ninhydrin reacts with free amines but if the amine is bound to some other molecule such as PEG which is further bound to nanoparticles, can Ninhydrin test still be used to estimate the amino...

11 December 2019 3,476 2 View

Conjugating cRGD with amine functionalized nanoparticles?

I have been working with nanoparticles with different functional groups. Usually its well known protocol for conjugating cRGD or RGD with COOH functional group but I have a few particles which are...

11 December 2019 3,688 3 View

Why is my XRD showing peaks s at ~7 and ~19 for Fe3O4?

I tried to synthesize oleic acid capped Fe3O4 nanoparticles and much to my surprise, they provide a strange XRD pattern (when started from 5 degrees (2 theta)). It shows a peak at ~7 (50% relative...

09 October 2018 720 8 View

Measuring size of a nanocube?

Would you suggest measuring the side of a nanocube and mentioning that in the paper or would you suggest writing the diagonal size of the particles ? Thanks in advance.

08 September 2018 8,599 6 View

Why do some particles in TEM images appear darker than other (for example see images attached)?

I am not sure why there is a difference in shade in particles analysed by TEM. Is it just because of the orientation of particles or is there more to it ? For reference I have attached a few...

08 September 2018 7,436 10 View

Sodium Gallate Synthesis?

How do I synthesize and purify Sodium Gallate?

06 July 2018 2,438 5 View

Basis for selection of Solvent?

I have read quite a bit of literature and I found that while synthesizing Fe3O4 nanoparticles, people have chosen different solvents mainly based on their boiling point to favor nucleation or...

06 July 2018 5,817 4 View

Why Sodium oleate is used in synthesis of Iron Oleate?

I was wondering about the role of sodium oleate in synthesis of iron oleate, are there any alternatives? And if we want to make iron linoleate for example, should we use sodium linolate or direct...

05 June 2018 7,758 9 View

Why unordered silica coating is done on Fe3O4 before adding Mesoporous silica onto it?

04 May 2017 8,806 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Are there any known solvothermal methods to synthesize MoS2 nanosheets using solvents other than water (like ethanol/ethanol-water mixture) ?

I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.

22 February 2021 9,246 1 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View