Hi everybody,
i am trying to isolate total RNA from plant rich in polyphenols. i read a lot of papers. Now i want to know about the best recipe of extraction buffer for RNA isolation. Plz suggest me the best chemicals with their final concentration (if possible with logic behind the activity of particular chemical)....
i have some question also---
why preheating at 65degree is necessary for CTAB extraction buffer?
what buffer is better, Tris or citrate buffer and why??
is any substitution of PVP in the market?
which detergent is better as alot of detergents are available ?
its an international platform for the researchers so i my expectations are high....
plz plz....discuss whatever you know..
Your suggestions will be highly appreciated!!!!!
Regards
Thanx kenneth!!!
I have no problem in RNA isolation but want to know the basics of all chemicals, as there are a lot of chemicals are avalable so what choice will be the correct. A protocol is never universal for all samples even in different parts of a plant. thats why i want to know all these bcoz i do not want to try and try.......
hope you will understand!!!!!
Regards
@Arun
dear arun, I also have worked on B. juncea, its a simple plant and easy to isolate RNA. I read a lot of papers since 1954 to 2011 to understand the nucleic acid isolation in deep. i think no one understand my questions or may not want to disclose their knowledge!!!!!
thanx a lot
Sorry to start this thread again. But now I have the same question, and no one is dare to answer. Moreover, google is also banned in Ch**a so good luck to me and everyone who is searching for it.
Fu** selfishness
Yes sir, i would also need amounts of ingrediants for CTAB buffer for RNA extraction.
Sorry about starting it again.. This is 8 years old thread.. You must be a professor by now
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