09 September 2012 1 2K Report

I did circularization of RNA and then synthsized cDNA using reverse primer. i got a controll pcr using both reverse and fwd primer while failed to get anything when use primers in reverse directions to get full length. I have some confusion to discuss here...

ii use SuperscriptIII in place of MMLV. Can this affect cDNA synthesis?

Is cDNA synthesized from circular RNA will be Circular?

is there any specific metod to foolow in cRTPCR ?

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Can anybody explain cRT-PCR? How much DNA free Total RNA (minimum) can be used to circularize RNA? I have only 20U T4 RNA ligase (Ambion) and 30ug Total RNA. (I don't want to perform CIP treatment.)

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