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Questions related from Muhammad Owais Shahid
Hello folks, I have read this somewhere: "Design primers in the conserved fragments if family genes need to be silenced. However If specific genes need to be silenced, non-conservative regions or...
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I have read some papers regarding RNA extraction from recalcitrant plants. Most of them have used modified CTAB method. But i couldn't find the exact amount of the ingredients used. These are the...
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The primer designing for this kind of RT and qPCR are very simple. But how to check it specificity and quality, as it is used for miRNAs.
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I have problem about the number of control samples in each of the steps of stem loop qRT. In the 1st step (Stem-loop RT), 2 types of controls are required [no RT control and no RNA control]. I am...
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I work with Rosa hybrida tissues, and total RNA extraction from it is so hard. There are other plant species which gives good quantity and intact total RNA. So, what is the reason behind the Roses...
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Hello experts, I want to clone a gene, which seems impossible to clone. First it is hard to amplify. After amplification (somehow) the vector is not ligating. I tried every possible vector...
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