Can anyone help with NGS platforms for digital gene expression analysis?

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How wobbling reduces number of tRNA required for polypeptide synthesis?

Please help me find right explanation...

08 September 2017 7,173 0 View

Illumina SE library

I am searching some material which can describe protocol used for production of mRNA library by single end sequencing by HiSeq2000 (illumina)P. I read all user guides provided by illumina on its...

05 June 2013 5,708 3 View

Protein 3D structure prediction.

I am new in the field of proteomics. I want to predict a 3d model for an amino acid sequence which has no entry in pdb ie., the protein is not characterized till now. I use a lot of software but...

03 April 2013 9,649 3 View

RACE PCR

I have 200 bp known fragment. for RACE PCR. I am using Clontech kit. I tried all the possible primers with UPM (with kit), but never got the desired band. I synthesized new RACE cDNA but still the...

08 September 2012 2,584 14 View

RNA circularization and cRT-PCR

I did circularization of RNA and then synthsized cDNA using reverse primer. i got a controll pcr using both reverse and fwd primer while failed to get anything when use primers in reverse...

08 September 2012 1,822 1 View

Circularization of RNA for cRT-PCR ?

Can anybody explain cRT-PCR? How much DNA free Total RNA (minimum) can be used to circularize RNA? I have only 20U T4 RNA ligase (Ambion) and 30ug Total RNA. (I don't want to perform CIP treatment.)

07 August 2012 6,962 1 View

RNA Extraction buffer recipe ??????????

Hi everybody, i am trying to isolate total RNA from plant rich in polyphenols. i read a lot of papers. Now i want to know about the best recipe of extraction buffer for RNA isolation. Plz...

06 July 2011 3,674 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View