Please help me find right explanation...
Please list the NGS platforms which were successfully used for gene expression analysis? I got only Illumina DGE tag profiling and ABI SOLID for the same work. Is there any report of GS FLX or...
04 May 2014 760 2 View
I am searching some material which can describe protocol used for production of mRNA library by single end sequencing by HiSeq2000 (illumina)P. I read all user guides provided by illumina on its...
05 June 2013 5,805 3 View
I am new in the field of proteomics. I want to predict a 3d model for an amino acid sequence which has no entry in pdb ie., the protein is not characterized till now. I use a lot of software but...
03 April 2013 9,747 3 View
I have 200 bp known fragment. for RACE PCR. I am using Clontech kit. I tried all the possible primers with UPM (with kit), but never got the desired band. I synthesized new RACE cDNA but still the...
08 September 2012 2,725 14 View
I did circularization of RNA and then synthsized cDNA using reverse primer. i got a controll pcr using both reverse and fwd primer while failed to get anything when use primers in reverse...
08 September 2012 1,930 1 View
Can anybody explain cRT-PCR? How much DNA free Total RNA (minimum) can be used to circularize RNA? I have only 20U T4 RNA ligase (Ambion) and 30ug Total RNA. (I don't want to perform CIP treatment.)
07 August 2012 7,069 1 View
Hi everybody, i am trying to isolate total RNA from plant rich in polyphenols. i read a lot of papers. Now i want to know about the best recipe of extraction buffer for RNA isolation. Plz...
06 July 2011 3,838 5 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Thanks
22 July 2024 1,143 3 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
