Please help me find right explanation...
Please list the NGS platforms which were successfully used for gene expression analysis? I got only Illumina DGE tag profiling and ABI SOLID for the same work. Is there any report of GS FLX or...
04 May 2014 630 2 View
I am searching some material which can describe protocol used for production of mRNA library by single end sequencing by HiSeq2000 (illumina)P. I read all user guides provided by illumina on its...
05 June 2013 5,708 3 View
I am new in the field of proteomics. I want to predict a 3d model for an amino acid sequence which has no entry in pdb ie., the protein is not characterized till now. I use a lot of software but...
03 April 2013 9,649 3 View
I have 200 bp known fragment. for RACE PCR. I am using Clontech kit. I tried all the possible primers with UPM (with kit), but never got the desired band. I synthesized new RACE cDNA but still the...
08 September 2012 2,584 14 View
I did circularization of RNA and then synthsized cDNA using reverse primer. i got a controll pcr using both reverse and fwd primer while failed to get anything when use primers in reverse...
08 September 2012 1,822 1 View
Can anybody explain cRT-PCR? How much DNA free Total RNA (minimum) can be used to circularize RNA? I have only 20U T4 RNA ligase (Ambion) and 30ug Total RNA. (I don't want to perform CIP treatment.)
07 August 2012 6,962 1 View
Hi everybody, i am trying to isolate total RNA from plant rich in polyphenols. i read a lot of papers. Now i want to know about the best recipe of extraction buffer for RNA isolation. Plz...
06 July 2011 3,674 5 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
