How wobbling reduces number of tRNA required for polypeptide synthesis?

Please help me find right explanation...

08 September 2017 7,173 0 View

Can anyone help with NGS platforms for digital gene expression analysis?

Please list the NGS platforms which were successfully used for gene expression analysis? I got only Illumina DGE tag profiling and ABI SOLID for the same work. Is there any report of GS FLX or...

04 May 2014 630 2 View

Illumina SE library

I am searching some material which can describe protocol used for production of mRNA library by single end sequencing by HiSeq2000 (illumina)P. I read all user guides provided by illumina on its...

05 June 2013 5,708 3 View

Protein 3D structure prediction.

I am new in the field of proteomics. I want to predict a 3d model for an amino acid sequence which has no entry in pdb ie., the protein is not characterized till now. I use a lot of software but...

03 April 2013 9,649 3 View

RACE PCR

I have 200 bp known fragment. for RACE PCR. I am using Clontech kit. I tried all the possible primers with UPM (with kit), but never got the desired band. I synthesized new RACE cDNA but still the...

08 September 2012 2,584 14 View

RNA circularization and cRT-PCR

I did circularization of RNA and then synthsized cDNA using reverse primer. i got a controll pcr using both reverse and fwd primer while failed to get anything when use primers in reverse...

08 September 2012 1,822 1 View

RNA Extraction buffer recipe ??????????

Hi everybody, i am trying to isolate total RNA from plant rich in polyphenols. i read a lot of papers. Now i want to know about the best recipe of extraction buffer for RNA isolation. Plz...

06 July 2011 3,674 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View