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Questions related from Avneesh Kumar
Please help me find right explanation...
09 September 2017 7,247 0 View
Please list the NGS platforms which were successfully used for gene expression analysis? I got only Illumina DGE tag profiling and ABI SOLID for the same work. Is there any report of GS FLX or...
05 May 2014 720 2 View
I am searching some material which can describe protocol used for production of mRNA library by single end sequencing by HiSeq2000 (illumina)P. I read all user guides provided by illumina on its...
06 June 2013 5,777 3 View
I am new in the field of proteomics. I want to predict a 3d model for an amino acid sequence which has no entry in pdb ie., the protein is not characterized till now. I use a lot of software but...
04 April 2013 9,718 3 View
I did circularization of RNA and then synthsized cDNA using reverse primer. i got a controll pcr using both reverse and fwd primer while failed to get anything when use primers in reverse...
09 September 2012 1,897 1 View
I have 200 bp known fragment. for RACE PCR. I am using Clontech kit. I tried all the possible primers with UPM (with kit), but never got the desired band. I synthesized new RACE cDNA but still the...
09 September 2012 2,695 14 View
Can anybody explain cRT-PCR? How much DNA free Total RNA (minimum) can be used to circularize RNA? I have only 20U T4 RNA ligase (Ambion) and 30ug Total RNA. (I don't want to perform CIP treatment.)
08 August 2012 7,040 1 View
Hi everybody, i am trying to isolate total RNA from plant rich in polyphenols. i read a lot of papers. Now i want to know about the best recipe of extraction buffer for RNA isolation. Plz...
07 July 2011 3,802 5 View
