I am planning to do a very preliminary experiment using a few representative solid cancer cell lines. Probably Three for each cancer types.
For Breast cancer, I am thinking to use MDA-231, MCF7 and maybe one HER2+ cell line. Since my research before is all about hematological malignancies, I would like to ask the experts' opinions about which cell lines are the representative or most commonly-used ones for the aforementioned three cancer types. Thanks
Aaron
Hi Aaron,
For breast cancer, MCF7 and 231 cell lines are good choice as they cover both luminal and triple negative subtypes, respectively. Iwould suggest SKBR cell line to represent Her2+.
best of luck with your research.
Mohamed
As the representative cell lines for breast cancer I would suggest to use MDA-MB231, MCF-7, SKBR3 and BT474 or some other cell lines mentioned in this nice publication: Article Holliday, D. L. & Speirs, V. Choosing the right cell line fo...
For representative Melanoma cell lines, you should used melanoma cell lines mutated on BRAF (such as A375), NRAF (such as SkMel2), NF1 (such as MEWO) or "triple negative" (such as CHL1). Read this recent publications (Luebker-Oncotarget 2017) for details of genomic alterations in a wide range of melanoma cell lines.
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