Hi!
Does anyone have a recommendation for a good phospho-parkin (Ser65) antibody to use for western blot? The cell signaling one didn't really work for me...
Thank you
Do you have a working positive control?
I need strategies to overcome food security challenges
23 April 2024 1,055 0 View
I need to take blood from Tegu Lizard and to extract the RBC following nuclei isolation, sonication and ChIP assay: appreciate any help: 1) which blood-collection tubes are recommended? 2) how do...
03 April 2024 9,657 0 View
Hi everyone, does anyone have some tips on how to quantify mitochondria morphology? I tried MINA but I just can't seem to get it right/don't understand how to use it....I would really appreciate...
02 April 2022 1,886 1 View
Does anyone have a recommendation for a tau antibody which is not mouse and can be a good axon marker? Can I use an antibody for phosphorylated tau? Thanks so much!
06 October 2021 2,649 0 View
Hi all! I need a neuronal marker to use in human iPSC. I need to look at the AIS so I need to know where the axon is...Therefore I don't want to use MAP2. The only tuj antibody we have in the lab...
26 July 2021 9,604 3 View
Hey, In a conference I attended, someone presented a CAS9 system that does not create a DSB in the classical location but rather further downstream than classical NGG adjacent location. I forgot...
25 August 2020 5,747 1 View
We conducted an MEG study aimed at understanding the neural mechanisms underlying hypnosis and our main finding is a significant reduction in occipital gamma power during hypnosis. We are a bit...
13 April 2020 131 4 View
I need to model pullout of a yarn in concrete matrix
29 December 2019 8,047 6 View
Hi, I am trying to conduct a test for the change in the proportion in certain variable named "Type D personality" (2 levels = Yes or No). I have 3 measurements (3 time points, same peoples) and...
18 December 2019 1,256 9 View
I have been staining yeast cells for analysis of cell cycle using SYTOX green and getting good results. The cells i'm now tiring to stain express GFP so SYTOX green wouldn't work as they emit...
06 June 2019 9,366 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View