Does anybody know about CAS9 variants that create a DSB in a location downstream to the PAM?

26 August 2020 1 6K Report

Hey,

In a conference I attended, someone presented a CAS9 system that does not create a DSB in the classical location but rather further downstream than classical NGG adjacent location.

I forgot to write anything down and this system seems useful for random mutations. Does anyone happen to know it by chance or something similar to it?

Hanna Alalam

CAS12a (cpf1) should do the trick it cuts downstream of its pam site as a staggered cut.

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