Does anyone have a recommendation for a tau antibody which is not mouse and can be a good axon marker? Can I use an antibody for phosphorylated tau?
Thanks so much!
I need strategies to overcome food security challenges
23 April 2024 1,055 0 View
I need to take blood from Tegu Lizard and to extract the RBC following nuclei isolation, sonication and ChIP assay: appreciate any help: 1) which blood-collection tubes are recommended? 2) how do...
03 April 2024 9,657 0 View
Hi! Does anyone have a recommendation for a good phospho-parkin (Ser65) antibody to use for western blot? The cell signaling one didn't really work for me... Thank you
08 August 2023 9,451 1 View
Hi everyone, does anyone have some tips on how to quantify mitochondria morphology? I tried MINA but I just can't seem to get it right/don't understand how to use it....I would really appreciate...
02 April 2022 1,886 1 View
Hi all! I need a neuronal marker to use in human iPSC. I need to look at the AIS so I need to know where the axon is...Therefore I don't want to use MAP2. The only tuj antibody we have in the lab...
26 July 2021 9,604 3 View
Hey, In a conference I attended, someone presented a CAS9 system that does not create a DSB in the classical location but rather further downstream than classical NGG adjacent location. I forgot...
25 August 2020 5,747 1 View
We conducted an MEG study aimed at understanding the neural mechanisms underlying hypnosis and our main finding is a significant reduction in occipital gamma power during hypnosis. We are a bit...
13 April 2020 131 4 View
I need to model pullout of a yarn in concrete matrix
29 December 2019 8,047 6 View
Hi, I am trying to conduct a test for the change in the proportion in certain variable named "Type D personality" (2 levels = Yes or No). I have 3 measurements (3 time points, same peoples) and...
18 December 2019 1,256 9 View
I have been staining yeast cells for analysis of cell cycle using SYTOX green and getting good results. The cells i'm now tiring to stain express GFP so SYTOX green wouldn't work as they emit...
06 June 2019 9,366 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View