Does anyone have a recommendation for an axonal marker?

More Gali Maor's questions See All

How to write a literature review on: food security impacts towards south african citizens suffering because of expensive food, energy crises ?

I need strategies to overcome food security challenges

23 April 2024 1,055 0 View

How to perform a ChIP assay from a Lizard blood?

I need to take blood from Tegu Lizard and to extract the RBC following nuclei isolation, sonication and ChIP assay: appreciate any help: 1) which blood-collection tubes are recommended? 2) how do...

03 April 2024 9,657 0 View

Recommendation of a good phosphorylated parkin antibody?

Hi! Does anyone have a recommendation for a good phospho-parkin (Ser65) antibody to use for western blot? The cell signaling one didn't really work for me... Thank you

08 August 2023 9,451 1 View

Mitochondria morphology analysis anyone?

Hi everyone, does anyone have some tips on how to quantify mitochondria morphology? I tried MINA but I just can't seem to get it right/don't understand how to use it....I would really appreciate...

02 April 2022 1,886 1 View

Does anyone have a recommendation for a tau antibody for staining the axon? An antibody that is not mouse?

Does anyone have a recommendation for a tau antibody which is not mouse and can be a good axon marker? Can I use an antibody for phosphorylated tau? Thanks so much!

06 October 2021 2,649 0 View

Does anybody know about CAS9 variants that create a DSB in a location downstream to the PAM?

Hey, In a conference I attended, someone presented a CAS9 system that does not create a DSB in the classical location but rather further downstream than classical NGG adjacent location. I forgot...

25 August 2020 5,747 1 View

Lower occipital gamma power during hypnosis: Not what you would expect. Or is it?

We conducted an MEG study aimed at understanding the neural mechanisms underlying hypnosis and our main finding is a significant reduction in occipital gamma power during hypnosis. We are a bit...

13 April 2020 131 4 View

How to model a cohesive interface between 2 materials in ADINA ?

I need to model pullout of a yarn in concrete matrix

29 December 2019 8,047 6 View

Non parametric test for repeates measures?

Hi, I am trying to conduct a test for the change in the proportion in certain variable named "Type D personality" (2 levels = Yes or No). I have 3 measurements (3 time points, same peoples) and...

18 December 2019 1,256 9 View

Looking for a cell cycle staining to use on yeast for flow cytometry that could be co-stain with GFP?

I have been staining yeast cells for analysis of cell cycle using SYTOX green and getting good results. The cells i'm now tiring to stain express GFP so SYTOX green wouldn't work as they emit...

06 June 2019 9,366 2 View

Semaphorin3A treatment: How to use semaphorine to repulse axons and improve dendritic harborization in my culture chamber?

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26 March 2024 2,632 0 View

What is an article influence score (AIS)?

23 January 2024 9,215 0 View

What are the future trends in AIS research?

بصفتك اكاديمي ومن خلال اطلاعك على البحوث والدراسات المتعلقة بنظم المعلومات المحاسبية ماهي الاتجاهات المستقبلية في ابحاث AIS

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How to retain viral injected fluorescence in paraffin embedded brain tissue in mice and rat?

We have injected GFP and td-tomato viral tracing injection in the mice brains to see axonal projections, and we are using tertiary butyl alcohol instead of ethanol for the dehydration process as...

09 October 2023 8,654 8 View

Why is my eNpHr (halorhodopsin) not showing up in my projections study, but my control virus is?

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01 August 2023 2,056 0 View

Are there any cell bodies in the IPL of the olfactory bulb?

Most descriptions of the olfactory bulb (particularly in mice) say that the axons of the projection neurons (mitral cells and tufted cells) run through the IPL, but I cant seem to find any mention...

24 July 2023 5,055 0 View

Fixation method for Tau antibody for Immunofluorescence ?

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09 July 2023 9,351 3 View

How to distinguish axon terminal from axon section?

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06 July 2023 9,942 0 View

How does the use of Blockchain technology help in accounting information systems in government institutions?

(AIS)

21 March 2023 3,202 3 View

IF staining for Neurons?

I'm looking for a specific and good marker to stain the neurons. I want to specifically use IHC. I want to analyze the morphology of the whole neuron therefore it has to mark not only the cell...

08 March 2023 4,114 2 View

Sylvain Rama

Hello Gali,

AnkyrinG is a classical AIS marker.

See for example Article Ankyrin G restricts ion channel diffusion at the axonal init...

Hilde Wohlfrom

Use NFH!! there is one fom Abcam (Chick) and from DSHB (mouse IgG1)

Andrew K Ottens

Beta-III Tubulin nicely stains the whole neuron , including the axon, which could give you a good co-staining to Ank-G staining of the AIS.