Does anybody know about CAS9 variants that create a DSB in a location downstream to the PAM?

Hey, In a conference I attended, someone presented a CAS9 system that does not create a DSB in the classical location but rather further downstream than classical NGG adjacent location. I forgot...

25 August 2020 5,617 1 View

How to model a cohesive interface between 2 materials in ADINA ?

I need to model pullout of a yarn in concrete matrix

29 December 2019 7,891 6 View

Looking for a cell cycle staining to use on yeast for flow cytometry that could be co-stain with GFP?

I have been staining yeast cells for analysis of cell cycle using SYTOX green and getting good results. The cells i'm now tiring to stain express GFP so SYTOX green wouldn't work as they emit...

06 June 2019 9,254 2 View

How porosity is measured when SEM images are provided?

i have SEM images and want to know how the porosity is measured?

12 August 2018 3,934 4 View

What are the procedures in gene annotation of a novel bacteria?

what are the procedures in gene annotation of a novel bacteria and which of the bioinformatics tools.

11 April 2018 2,448 4 View

Does the concentration of the extract influence the GC/MS analysis?

how the concentration of the extract analyszed can influnece on the quality of the GC/MS analysis. Should I use low concentrations or high concentrations?

14 January 2018 8,209 5 View

Extract RLC circuit from S-parameter touchstone file?

28 September 2017 5,255 3 View

RLC Equivalent circuit of slotted microstrip patch antenna

Hi, I have designed a micro-strip patch antenna on HFSS. How to extract R-L-C equivalent circuit from S parameter? Can you help me please..??.. Thanks in advance.

14 August 2017 185 1 View

Is it possible to optimize fuel efficiency of Mini vans with add-on Spoiler and Diffuser?

I am studying external aerodynamics of ground vehicles. I want to do a project on reducing fuel consumption of mini vans by using spoiler and diffusor. Is it possible?

14 June 2016 6,231 4 View

Does Aspergillus carbonarius consume ammonia to reduce the pH?

Read 5 answers by scientists to the question asked by Uriel Maor on Dec 6, 2015

05 December 2015 7,410 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View