Does anybody know about CAS9 variants that create a DSB in a location downstream to the PAM?

Hey, In a conference I attended, someone presented a CAS9 system that does not create a DSB in the classical location but rather further downstream than classical NGG adjacent location. I forgot...

25 August 2020 5,617 1 View

Looking for a cell cycle staining to use on yeast for flow cytometry that could be co-stain with GFP?

I have been staining yeast cells for analysis of cell cycle using SYTOX green and getting good results. The cells i'm now tiring to stain express GFP so SYTOX green wouldn't work as they emit...

06 June 2019 9,254 2 View

How to stain for slow vs fast muscle fibers in PFA fixed tissue?

09 August 2017 7,346 3 View

Fermi level changes with applied electric field?

Hi people, I dont understand why when we apply the electric field to the metal Ef remains the same. Ef as translation energy of electrons remains the same but we accelerate the electrons with...

08 March 2016 1,747 3 View

What does it mean the high real part of refractive index of metal?

in concept of material structure.

21 February 2016 6,918 1 View

What publications on classical mesoscopic systems can be found?

The theory of mesoscopical systems can be divided into two parts: theory of mesoscopic quantum systems and the classical theory of mesoscopic systems. In a quantum mesoscopic considered electronic...

24 December 2015 5,261 3 View

Does Aspergillus carbonarius consume ammonia to reduce the pH?

Read 5 answers by scientists to the question asked by Uriel Maor on Dec 6, 2015

05 December 2015 7,410 4 View

Why in derivation of the equations of continuum mechanics the model of material point is used?

Model material points cannot be used for describing the motion of macroscopic bodies. The derivation of the equations of continuum mechanics based on the use of limit theorems, in which the size...

11 October 2014 3,519 17 View

Can you use equations of motion not for material points but for equations of mesoscopic particles?

In mesocsopical systems, interactions described with action fluxes in a mechanical system are flow of impulse. (See attachments.) Lev

20 August 2014 6,438 2 View

How to change the pH of PBS to pH=9, and pH=11?

For pH stability experiments by NMR, I need to change the pH of PBS from 7.4 to 8 and to 11. Has anybody done something similar?

22 January 2014 4,563 13 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View