Is it reasonable to use half your dataset to carry out exploratory analysis leading to specific hypotheses, and then test these using the other half?

There is a clear distinction between exploratory and confirmatory research. In exploratory research, we explore our data to discover interesting trends or associations, perhaps by analysing it in...

25 July 2019 9,588 4 View

How to add published research directly from the publication web site?

I have published articles and a PhD thesis which are not listed here. How can I add the link to the publication site here?

17 May 2019 4,398 2 View

What is the experience with Duck Duck Go as a search engine?

Concern about data mining and the uses to which the mined data are put suggest that privacy is not just a matter of personal sensitivities. It may, at least partially, be a social duty if it can...

15 September 2018 6,988 4 View

How does taking multiple measurements under the same condition affect experiment power and effect sizes?

In our research group we carry out psychophysics experiments that involve taking multiple measurements, e.g. reaction times or correct detections, under the same combination of variables. I...

01 August 2018 7,372 7 View

Is there a source to learn which cell lines bind RGD (are integrin-expressing)?

Hello, I was wondering if anyone knows of a resource which lists cell lines (common ATCC.org ones) that bind RGD (and ones that don't). Ultimately, we'd like to obtain a cell line that has high...

25 March 2018 8,053 9 View

Can anyone suggest a way to quantify viral RNA in the pg range other than PCR or Agilent's technology?

Viral genome is too large for Agilent's technology, and I do not want to use RT-qPCR. I am thinking mass spec, but would rather get something I can do in our labs.

08 March 2018 192 3 View

Looking for help building a layered structure in FDTD

I'm looking to build a layered quarter wave stack structure in a FDTD simulation. I've tried both 2-D and 3-D and it hasn't worked. I'm able to do a block of solid material but not layers. The...

10 August 2017 6,385 13 View

Is it appropriate to use a weighted sample size in calculating standard errors and conducting significance tests?

SPSS' regression procedure, when conducting a weighted analysis (to provide unbiased estimates of population parameters) bases standard errors and significance tests on the weighted N. For a...

02 January 2017 4,722 4 View

I'm trying to model choices over time. Does SPSS have a statistical procedure for this analysis?

This administrative study investigates the choices students make in a menu of choices curriculum design. Given a gateway course, where do they go from there? And then from there, etc.? I will have...

10 September 2015 4,881 7 View

How can I use an Instron for soil penetration resistance measurements?

12 June 2015 6,962 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View