Jim Mcmichael

5 Questions 6 Answers 0 Followers

Questions related from Jim Mcmichael

Can anyone suggest a way to quantify viral RNA in the pg range other than PCR or Agilent's technology?

Viral genome is too large for Agilent's technology, and I do not want to use RT-qPCR. I am thinking mass spec, but would rather get something I can do in our labs.

09 March 2018 342 3 View

Can anyone suggest a housekeeping protein for RAW 264.7 cells?

Beta-actin is up-regulated during LPS stimulation, and I can't seem to find any consistent proof of a HKP that works in RAW cells. We need a good normalizer for western blot analysis.

03 September 2015 3,230 4 View

Does anyone know a good way to separate fungal conidia and hyphae that are clumped together to make them easier to count?

The cells in question have been fixed in 1% paraformaldehyde. The problem is that the hyphae intertwine upon germination, and it's difficult to observe individual conidia germination. I know that...

08 March 2014 2,209 16 View

When to remove a trplicate sample before analysis of mRNA gene expression.

Can anyone offer any advice on how to decide when to remove a triplicate from the endogenous control or the target genes before analysis? I am interpreting the raw data using excel. It is an mRNA...

16 April 2013 9,956 2 View

Quality DNA from swabs samples

Can anyone suggest a good method or kit to extract very low amounts of quality DNA from fungal swab samples? It seems like the less conidia we start with, the worse the protein contamination...

20 March 2013 1,818 3 View