Protocol for the detection of ROS and RNS in mouse lung cell isolates using flow cytometry?

26 August 2016 1 7K Report

Can anyone share a protocol to detect ROS and RNS in the immune cells isolated from mouse lungs, using flow cytometry.

NOTE: Fixing of these cells before running them on the flow-cytometer is absolutely necessary for me.

Thanks for the help.

Hi Gustavo, I've used CellRox Green and DAF-FM (Life Tech.), both of which haven't worked in my multi-channel flow cytometry.

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Science method

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