Assuming that the standard curve drawn to derive the equation is in the units of µg/mL.

Y = 0.0057x + 0.0635

In this equation, we have found that y= 0.39,

0.39 = 0.0057x + 0.0635

0.39 – 0.0635 = 0.0057x

0.3265 = 0.0057x

x = 0.3265/ 0.0057

x = 57. 28 µg/mL

This implies that in 10mg/mL of extract we have 57. 28 µg/mL of quercetin.

In 10 mg we have 57.28 ug quercetin

For 1000 mg i.e. 1g = 57.28 *1000/10

= 5728 µg

= 5.728 mg quercetin/g

Rishibha Gupta Thank you so much for providing clear answer.

The Total Flavonoid Content (TFC) for each sample variation can be calculated using Equation 1 as miligrams of Quercetin Equivalent (QE) per gram of anti-atherosclerotic herbs by multiplying the quercetin concentration of the sample in ppm (C) by the volume of the solution in mL (V), and then dividing it by herb mass

Sorry, I did not understand your concept. Can you please elaborate it in simple meaning. Thank you for giving me response. Ismahil Adekunle Adeniyi