For purification of carbohydrases (carbohydrate degrading enzymes) from bacterial cell free supernatant (CFS), which Ion exchange resin can be used?
Are there prepacked columns available for the same, if not , please suggest the resin to be used?
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...
23 June 2024 8,785 5 View
Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...
19 June 2024 6,595 3 View
I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...
07 June 2024 203 2 View
Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...
04 June 2024 1,501 0 View
I wanted to know the value of total flavonoid content in mgQE/gm. Total volume I kept in well plate is 200 µl in which extract was 20 µl. Anyone please help me with this problem
10 May 2024 5,657 4 View
Amount of fruit powder used 0.15g; final volume of the extract was 100 ml and I have done my experiment in flame photometer. I did not know how to convert 45ppm in mg/100g. Please help me?
10 May 2024 3,173 0 View
Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...
17 April 2024 8,522 3 View
Which is probably a better and efficient formulation for the application of Pre-emergent herbicide? A) Dry formulation B) Liquid formulation?
11 April 2024 5,156 1 View
Hey all I wish to know the change in expression level of certain genes post treatment with drug. For this can I isolate RNA after 24hrs of treatment? Should I wait till 48 hours? Or should I...
06 April 2024 9,082 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
My thesis includes variables of inflation rate (monthly), interest rate (weekly), exchange rate (daily), and crude oil production (monthly), as well as imports and exports (annually). The period...
27 July 2024 7,384 5 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,292 4 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...
23 July 2024 1,672 0 View
https://en.wikipedia.org/wiki/Viscosity
21 July 2024 3,089 4 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,461 1 View