Krishna Chinta

3 Questions 2 Answers 0 Followers

Questions related from Krishna Chinta

How much of a difference will 5% FBS and 10% FBS make in cellular responses of mouse BMDM's?

I was wondering if culturing mouse BMDM's in 5% FBS vs 10% FBS will have significant differences in cell responses such as gene expression, cytokine production, etc.

09 October 2018 668 3 View

Protocol for arginase assay in mouse macrophages ?

Can anyone share a protocol for measuring arginase activity in mouse peritoneal macrophages? Any information, detailed protocol or a catalog number for a kit that works will be really...

20 March 2017 9,970 1 View

Protocol for the detection of ROS and RNS in mouse lung cell isolates using flow cytometry?

Can anyone share a protocol to detect ROS and RNS in the immune cells isolated from mouse lungs, using flow cytometry. NOTE: Fixing of these cells before running them on the flow-cytometer is...

26 August 2016 6,886 1 View