What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Disappearing Coomassie Bands?

Suddenly my Coomassie staining has produced strange results (see photo attached). The past 8 gels all share the same trends: the bottom half of the gel fades out and is absent of any bands. I...

19 December 2020 3,619 1 View

What is the maximum passage number of SKOV-3 and SKOV-3-Luc cells?

Hello, for my experiment I'm working with SKOV-3-Luc cells, which are SKOV-3 cells stably/continuously expressing the firefly luciferase enzyme. These cells are obtained by the transfection of...

25 November 2020 3,219 3 View

Masters thesis subject - International humanitarian law/ International criminal law?

Hello I am a master's student and I'm searching for a current debate in the field of international humanitarian law or international criminal law. Is there somebody who can help me with this?...

08 November 2020 1,571 13 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

29 September 2020 7,778 2 View

Can anyone tell me which species (or higher taxon) this is?

We found this small, maybe parasitic, crustacean in the North Sea offshore. The posterior body part is missing, but it looks quite characteristic. There is double-hooked rostrum, 2 antennae...

08 April 2020 6,842 9 View

Can I use time as a predictor variable in a panel regression?

Is it possible to generate a new variable "phase" that is based on the time the participants were questioned? I generated this new variable by dividing the time into three phases (beginning,...

30 March 2020 3,756 6 View

Dielectric constants of organic:aqueous mixtures, is linear addition a valid approximation?

I would like to compute dielectric constants of organic:aqueous mixtures. Would linear addition of the dielectric constants of the pure solvents give reliable results? For example, for a volume...

26 March 2020 5,939 3 View

Which SPSS test should I use?

For a small project, I have to calculate a few things via SPSS. However, I am not sure which tests to use. I have multiple variables. Independent variable: categorical variable with two levels....

12 March 2020 7,188 3 View

Is there a device which can dillute a gas-sample before it enters a continious-flow gas-analysator?

I want to measure gaseous NH3 emitted from slurry with the gas-analysator G2508 from Picarro. I expect the concentration to be ten and more times higher than the operating range of the...

02 October 2019 8,276 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View