Is it possible to share either input files or output files between users of Visual MINTEQ?

I am working with undergraduate students in an online mode (due to the COVID-19) instead of in-person as I have in past semesters. A few are having trouble running their samples, which they...

15 March 2020 8,303 2 View

Can I mix trypticase soy broth and nutrient agar together?

I am a non-microbiologist doing some microbiology work. I have plenty of dehydrated media for both nutrient agar and trypticase soy broth. If I need to make some trypticase soy agar, without...

12 November 2019 2,893 6 View

Is there an acceptable pH range for vaccines? Is there an acceptable range defined by FDA or other regulatory agencies?

In the case that vaccine antigen is more stable at lower pH, how low the pH can go based on the experience of existing marketed vaccine products? Where to find the pH information of marketed...

08 April 2019 286 3 View

What issues about complaint, safety and health for my research?

I plan to make an article on the topic of complaints, safety and health. but I don't know what title or issue I have to raise, because I am a beginner in this field of study. Please give me a...

20 February 2019 5,964 4 View

Negative correlation interpretation?

How would you describe the relationship between two scores with an r obtained of -.312, with a p value = .000?

25 November 2018 6,909 7 View

Can we use Pearson's r for determining the relationship of two variables with instruments using the likert scale?

Our thesis research has the following questions: 1. What is their level of (variable a)? 2. What is their level of (variable b)? 3. Which of the five components of (variable b) relate to...

04 November 2018 2,825 4 View

Does anyone have a copy of the questionnaire and all the psychometric properties of workplace deviance?

This particular scale is needed for my thesis. Thank you very much.

09 September 2018 6,117 3 View

I got a 20% response rate on my emailed survey with questionnaires and written qualitative questions. Might the qualitative analysis be publishable?

Even though qualitative data aren't generalizable, would the low response rate (but n=65) bias the results anyway?

24 July 2018 6,554 8 View

What would keep short DNA oligonucleotide from migrating away from well in gel electrophoresis?

I have a sample that is a mix of DNA (~100bp) and maltodextrin sugar with some processing. I need to show the DNA size via gel electrophoresis (GE), but the DNA remains smeared at the top of the...

05 March 2017 7,703 6 View

Is it necessary to know the sulphur content of an activated carbon?

01 February 2017 6,763 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View