I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all seemed to have taken the plasmid. Today, as I checked my cells, I noticed that one of them got contaminated while the other was fine. Do you know what could be a possible reason for this phenomenon? If it were the media, should not both plates be contaminated? Could there be a problem with the virus itself? My NTC plate is fine, but two of the three shRNAs got contaminated. Please let me know if you have faced similar issues with your lentiviral transfection and how you went about tackling them. Thanks! :)

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