I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the Fisher 150 Homogenizer (15-340-167), as well the 7mm Fisher saw-tooth probe (15-340-174). If anyone has experience using these products or knows of another product that will work, please let me know.
I have used a Fisherbrand handheld homogenizer for RNA extractions from a variety of tissues (including lung) with good success.
I would suggest using a 2.0 mL cryovial rather than the 1.5 mL Eppendorf tube as the bottom is much less curved and the tube does not taper.
Thanks for your answer John Hardy Lockhart
What do you use to clean the probe between uses? I'm think RNAse Zap and Nuclease-free water?
Sommer Jarvis
I typically do a few rinses in 50 mL tubes between use with brief activation of the probe in each:
After this I disassemble the probe to check/remove remaining tissue. Reassemble the probe, and dip the tip in Trizol/Qiazol (which is what I perform my homogenizations in).
I only use RNAse Zap on the workspace and probes before I begin the first homogenization. You can definitely include that as an extra step during the cleaning between samples if you want, just make sure you follow it with a water rinse.
I've attached my complete protocol if you are interested.
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