I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of successful electroporations is very low.

Since I work on HIV-I infected patients, their consequent variability is of Course taken under consideration. However, the rate of successful electroporations is still too low to what is expected.

Are there any tips or advice regarding viral mRNA electroporation I can get? It´ll be much helpful and appreciated.

Thanks in Advance!

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