3 Questions 27 Answers 0 Followers
Questions related from Patrick Corbin Mullen
Is it possible for PCR products to migrate differently due to secondary structure? I am performing RT-PCR to analyze transcripts in control and patient fibroblasts. When performing RT-PCR I...
06 June 2019 2,792 17 View
I have been trying to use PCR to amplify cDNA using standard Taq polymerase. I am able to amplify beta actin, but not my target. Unfortunately, the Tm for my two primers is somewhat far apart...
01 January 2019 8,285 6 View
I have a curious result where my protein of interest is significantly down-regulated (90%) in knockout cells by Western blot, but appears to be unchanged on IF. I have repeated the western result...
01 January 2019 9,448 0 View