Patrick Corbin Mullen

3 Questions 27 Answers 0 Followers

Questions related from Patrick Corbin Mullen

RT-PCR secondary structure?

Is it possible for PCR products to migrate differently due to secondary structure? I am performing RT-PCR to analyze transcripts in control and patient fibroblasts. When performing RT-PCR I...

06 June 2019 2,793 17 View

How should a PCR annealing temperature gradient work?

I have been trying to use PCR to amplify cDNA using standard Taq polymerase. I am able to amplify beta actin, but not my target. Unfortunately, the Tm for my two primers is somewhat far apart...

01 January 2019 8,286 6 View

Why is signal for knockout cells drastically reduced (90%) on WB but not IF?

I have a curious result where my protein of interest is significantly down-regulated (90%) in knockout cells by Western blot, but appears to be unchanged on IF. I have repeated the western result...

01 January 2019 9,449 0 View