I would like to have as a control for my experiments, a non cancerous cell line of bone origin. Hfob is a an alternative but didn´t find someone who cultivates this cell line in Brasil.
Thanks for help.
Mozart
You may want to contact the authors of:
https://www.ncbi.nlm.nih.gov/pubmed/18979172
All are in Brazil.
And note that "Hfob" is an alternative name for "Hfob 1.19", not a separate cell line. See:
http://web.expasy.org/cellosaurus/CVCL_3708
Vc pode entrar em contato com o Banco de Células do Rio de Janeiro (é só digitar no Google).
Acho q nós temos esta linhagem em nosso Banco de Células, mas, como não a usamos há muito tempo, seria necessário descongelar e caracterizar extensamente. Infelizmente, não temos apoio técnico para isto. Em todo caso, aqui vai meu Fone:
11-2648-0226
Boa sorte Mozart!
Thank you Prof. Sogayar and Bairoch. I did try to contact some o f the authors but got no answer or from another group they didn´t cultivate the cell line anymore.
I didn´t notice, however, Prof. Sogayar was in that paper, who I actually know from my times as a undergrad student. Kind as she always was to me, she left me her contact.
Prof. Sogayar, I usually buy cell from the Cell Bank in Rio de Janeiro but this one is not in their catalog. I will contact them as you suggested just to be a hundred per cent sure.
I conduct cytotoxic screening with osteosarcoam cell lines (U2O, MG63 and Saos2) and the hfob would be a control for a normal osteoblast cell line. I could use some mouse cell lines (i.e. MC3T3-E1 ) but referees are getting difficult about using mouse cells as a control. So I though hfoB would be an alternative.
Would you or Prof. Bairoch has any suggestions?
Thank you.
Can't really help you in term of suggestions of what would be a good control as a normal osteoblast cell line. I just stumbled on a paper on a cell line OPC1, I was not aware of yet:
https://www.ncbi.nlm.nih.gov/pubmed/10491220
Which seems to be distributed:
https://www.abmgood.com/Immortalized-Osteoprecursor-Cells-(OPC1)-T0230.html
But can confirm that Hfob 1.19 is not in the BCRJ catalog as I monitor their site to make sure the Cellosaurus represent the current offering of their cell lines. Their full list is at:
http://bcrj.org.br/catalogo/lista-completa/
Best
Amos
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