Pepsin, pancreatin, oxbile,, are they autoclavable? how to sterilize them?

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Why citations are not recorded on my profile researchgate web?

Dear colleges , I would appreciate if you have a time to help. I received mails told me there are numbers for research articles. These citations are not recorded on my profile. Regards, Sherif

09 July 2024 6,195 1 View

How the ventilator generates positive pressure in PSV?

I read that "PSV assists the patient's effort by delivering a positive pressure during inspiration. This reduces the work required to expand the lungs and overcome airway resistance, making...

20 April 2024 1,660 1 View

How to increase the efficiency of doxorubicin entrapment in albumin nanocarrier?

I obtained up to 30% encapsulation efficiency by using the desolvation synthesis method, in different pH level(7-8/5) with 8% glutaraldehyde as a cross-linker, this work has been repeated many...

19 April 2024 8,496 3 View

How can I induce type 2 diabetes in C57 female mice?

Hi everyone, I tried many published protocols to induce type 2 diabetes in c57 female mice using high fat diet for 1 month then STZ 40 mg for 4 days or 50 mg for 5 days followed by 10% sucrose in...

18 April 2024 4,681 2 View

Is rhodamine B can be used to prepare chlorophyll ‘a’ concentration?

I want to measure spectra of Chlorophyll ‘a’ in the lab , Is rhodamine B can be used to prepare chlorophyll ‘a’ concentration with the aim to measure spectra in lab using spectrophotometer?

14 April 2024 7,550 0 View

How can I create a citation/citation for a specific research found on the website?

كيف يمكن عمل استشهاد/ اقتباس لبحث معين موجود علي الموقع ؟

06 April 2024 9,899 1 View

Understanding PCA plot for MD simulations ?

In this figure, a pca plot is shown for different protein , protein complex systems depicting the essential subspace for these systems. I want to know how can I interpret these plots ? What...

31 March 2024 9,951 3 View

Why reflectance is greater than 1 in some cases?

I prepared different concentrations of chl-a using Rhodamine B (CHClN₂O₃) as indicator, my aim is to measure the spectra for each concentration trying to find relationship between them, the...

19 March 2024 2,147 0 View

what the applications of Tag SNPs selection and Haplotype Blocks are in the context of rare pathogenic variations?

Hi, Could somebody tell me what the applications of Tag SNPs selection and Haplotype Blocks are in the context of rare pathogenic variations in a Mendelian disease. Appreciate your...

17 March 2024 4,034 0 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

How to ask to correct the Incorrect Claim regarding the writer of Article that I was involved in?

Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...

14 July 2024 9,579 2 View

Is it necessary to autoclave every vessel used in DNA isolation?

DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?

10 July 2024 7,526 3 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

Can all postbiotics survive the gastric journey or is it again strain-specific or will depend the type of postbiotic?

General understanding is that postbiotic particularly those that are heat-treated are stable to heat and their activity remains stable during gastric journey. Wondering if this is true for all...

25 June 2024 3,133 3 View