Hi everyone,
I tried many published protocols to induce type 2 diabetes in c57 female mice using high fat diet for 1 month then STZ 40 mg for 4 days or 50 mg for 5 days followed by 10% sucrose in drinking water to avoid hypoglycemia however, the mice were resistance when I gave 40 mg stz for 4 days and died when I inject 50 mg for 5 days (these 2 protocols were used in multiple papers including nature ones). Any suggestions?
Thank you in advance
Sherif
The stages of diseases are 1,2,3, it is better to control all diseases in the first stage otherwise they become severe and insulin resistance is 2 stages.
Metabolic disorders occur due to our bad habits of eating process. Like food, water, and liquids, etc. Due to mutations in all our food in saliva, the imbalance of minerals in our body parts and some parts of our body and endocardial gland malfunction. If we do not change our habits, then we will feel difficulties, and these habits, abnormalities, and things are going to our inborn children. And problems are increasing day by day. We are seeing that our inborn children suffer from metabolic disorders from birth, like diabetes, etc. These habits, abnormalities, and genes we cannot correct with any medicine, substitutes, or research like stem cells, gene therapy, etc. "Habits Is a Second Nature “I request all people. Please read my book and follow the eating rules. Then we can achieve victory over metabolic disorders. I welcome all of you at these links:- https://www.lulu.com/shop/innovator-pramod-stephen/your-health-is-in-your-mouth/paperback/product-zgvvk6.html?page=1&pageSize=4 https://www.flipkart.com/aapka-swasth-aapke-muh-me/p/itm22edae101e74a?pid=9789393385543
Every disease has 3 stages 1st stage diabetes 1, In 1st stage it starts to damage the nephron mussels etc. 2nd diabetes 2, In this stage body, gets insulin resistance and 3rd stage the kidney, liver, eyes, and ears, which are affected badly and swellings in any part of the body mostly the legs affected and it becomes tough and lastly, death occurs in young stage. It is better to control metabolic disorders and save the next generations every person must read my book "Your Health Is In Your Mouth"
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