No title

31 December 2019 8,862 3 View

Which citation style include almost every information. like publishing house for the journal too?

Which citation style include almost every information i.e. most detailed including every information on the journal like publishing house for the journal too?

10 November 2019 1,532 3 View

What's the difference between TOP10 and JM109 Ecoli strains?

Whats the difference between TOP10 and JM109 Ecoli strains? Is that the TOP10 able to express GFP tagged protein and can be visualized under blue light if we are doing cloning, then what makes it...

09 October 2019 3,378 3 View

What would be life time difference between soluble protein and amyloids?

How would be amyloid and soluble protein life time difference? if we have cells in which we tagged protein with eGFP and then FLIM image them and compare within cell the life time. How would it be...

08 September 2019 9,659 1 View

FLIM data analysis?

I got a ".lif" file from confocal FLIM imaging, I have 0 min and 60 min data with 15 min interval. I want to show lifetime for these timepoints. How can I start with analysis and making figures...

08 September 2019 2,123 1 View

Would ZnF motif (in my protein of interest) get destroyed by TCEP during labeling of Fluorescent dye to Cysteine in protein?

My protein has ZnF motif, would using of TCEP destroy this and have effect on RNA binding as I need to use it for the Cys labelling with fluorescent dye.

02 March 2019 3,801 4 View

Should I add PI, PMSF and Peptstatin during protein purification in all buffers?

Should I add PI, PMSF and Peptstatin during protein purification in all buffers like resuspension, wash elution buffer in all steps or just until first wash buffer and second wash buffer as well...

31 December 2018 2,155 11 View

mEOS3 tagged protein concentration by spectrophotometer?

I have protein tagged with mEOS3 and want to check it's concentration after purification with spectrophotometer. How can I do that? What wavelength I should use and what Extinction coefficient to...

31 December 2018 8,749 2 View

No title

10 November 2018 9,291 3 View

What does tis sentence mean?

The abc (region of a protein) of xyz (protein name) binds the 50kDa ZYX (protein name) with dissociation constant (kDa) of 30nM. Can one explain what does this dissociation constant mean and what...

08 September 2018 768 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View