14 Questions 14 Answers 0 Followers
Questions related from Prem Kumar G
Hi everyone, Im trying targeted gene editing in citrus protoplast through PEG-mediated transformation. Im using sgRNA+Cas9 RNP complex for DNA free genome editing. But im not successful in...
07 July 2019 1,435 1 View
I am going to check my gRNA efficiency by in vitro digestion of DNA with Cas9. I read the protocol from NEB website but have some doubts regarding that. I want to know whether i should do in vitro...
06 June 2018 9,889 0 View
Dear All, I want to design sgRNA target sequence and insert into scaffold portion already present in pRGEB31 binary vector. Any experimental protocol for that? I want to knock out a disease...
06 June 2018 4,869 2 View
Dear Researchers, I am doing transformation in cotton plant. Now I want to check GFP expression in transformed tissue. Can anyone suggest a standard protocol for preparing tissue sample to...
11 November 2016 1,380 5 View
Dear Researchers, Im trying vacuum infiltration for seed transformation of a recalcitrant plant. I tried different time durations of vacuum infiltration (1 min to 10 min), culture density...
09 September 2016 6,799 3 View
Dear Researchers, Is it possible for cotton seedlings (10 days old) to produce antioxidant enzymes in cotyledons and other parts under short duration (6h,12h and 24h) of salt and drought...
07 July 2016 4,927 3 View
Hi Researchers, Currently I am doing 2DGE to identify differential protein expression (unknown) in plant tissues under various abiotic stress conditions. I am following phenol extraction...
06 June 2016 371 4 View
Dear Researchers, I was preparing to run 2nd dimension (SDS PAGE) after finishing 1st dimension (isoelectric focusing). After finishing everything perfect, i was sealing top of the IPG strip...
04 April 2016 528 13 View
Dear all, I have planned to study differential expression of proteins through 2-D electrophoresis (not any target protein) in 7 days old cotton seedlings under different abiotic stress (NaCl,...
04 April 2016 8,198 7 View
Currently I am doing SDS PAGE. After adding all solutions, polymerization did not occur. I never encountered this problem previously. I prepared every solution freshly with correct procedure. I...
03 March 2016 3,826 6 View
Hi researchers, for the first time i am going to do silver staining of SDS PAGE. can anyone suggest me a good protocol for doing that? Thanks in advance.
03 March 2016 1,945 6 View
Does anyone know the procedure for visualizing GFP expression in transgenic plant tissues?
01 January 2016 6,418 3 View
I have gold silver and nanoparticle samples (synthesized using aqueous extract of plant). now i want to determine their antioxidant activity by DPPH assay. Does anyone have experience in doing...
05 May 2015 4,265 2 View
Which one I have to use as a blank for protein quantification in bradford assay whether the buffer (in which I extracted protein) or bradford reagent?
05 May 2015 1,022 4 View
