22 Questions 12 Answers 0 Followers
Questions related from Aron Sd
I am looking for a non–fluorescent mutant of GFP, which should be mutant folds to a stable, native‐like structure but lacks fluorescence, should be able to tag with protein and express purify...
01 January 2020 8,985 3 View
Which citation style include almost every information i.e. most detailed including every information on the journal like publishing house for the journal too?
11 November 2019 1,657 3 View
Whats the difference between TOP10 and JM109 Ecoli strains? Is that the TOP10 able to express GFP tagged protein and can be visualized under blue light if we are doing cloning, then what makes it...
10 October 2019 3,571 3 View
I got a ".lif" file from confocal FLIM imaging, I have 0 min and 60 min data with 15 min interval. I want to show lifetime for these timepoints. How can I start with analysis and making figures...
09 September 2019 2,314 1 View
How would be amyloid and soluble protein life time difference? if we have cells in which we tagged protein with eGFP and then FLIM image them and compare within cell the life time. How would it be...
09 September 2019 9,773 1 View
My protein has ZnF motif, would using of TCEP destroy this and have effect on RNA binding as I need to use it for the Cys labelling with fluorescent dye.
03 March 2019 3,931 4 View
Should I add PI, PMSF and Peptstatin during protein purification in all buffers like resuspension, wash elution buffer in all steps or just until first wash buffer and second wash buffer as well...
01 January 2019 2,363 11 View
I have protein tagged with mEOS3 and want to check it's concentration after purification with spectrophotometer. How can I do that? What wavelength I should use and what Extinction coefficient to...
01 January 2019 8,891 2 View
I am looking for a Gel Doc style open UV light box which can be sued for the gel cut for gel extraction cloning purpose. What’s the best platform available which should expose minimum UV to hands...
11 November 2018 9,414 3 View
When I am making Wash buffer where I am using 50mM NaPo Buffer, 300mM NaCl and 10uM ZnCl2 (Stock 1mM ZnCl2 pH ~2 partial cloudy), Now when I make this wash buffer the solution is cloudy at pH 7.5,...
09 September 2018 8,156 1 View
The abc (region of a protein) of xyz (protein name) binds the 50kDa ZYX (protein name) with dissociation constant (kDa) of 30nM. Can one explain what does this dissociation constant mean and what...
09 September 2018 923 1 View
What are the important proteins we need to do croEM in cell division, chromosome separation or microtubule role in cell division context study to get detailed insight? Please list them.
09 September 2017 5,030 0 View
Can anyone please explain how to interpret a MT related kymograph (like polymerization depolymerization or any other parameter etc) with figures. How a kymograph look if a kinesis depolymerize MT...
08 August 2017 395 1 View
I have taken CFP and YFP fluorescent images with Nikon microscope which has Slidebook image format, now I want to take these images for the publication purpose process in Adobe photoshop then...
07 July 2017 2,802 1 View
Which free software one can use to check grammar, and plagiarism for articles or review writing? This should be free or cheap and have comprehensive details on grammar, plagiarism and writing style.
06 June 2017 8,167 2 View
https://www.ncbi.nlm.nih.gov/pubmed/25181302 http://www.nature.com/nmeth/journal/v6/n12/full/nmeth.1401.html If anyone has followed and used method to delete two proteins with auxin based degron...
06 June 2017 7,088 1 View
I have graph in prism file and want to put that lets say in figure section (C) in adobe illustator and fit it in size for C section. Can any body show step by step how to go about that. Thanks.
04 April 2017 8,424 6 View
Hi, I want to stain Yeast cells with Rhodamine Phalloidin (Amanita phalloides) Cat. # PHDR1 (http://www.cytoskeleton.com/pdf-storage/datasheets/phdr1.pdf) I want the protocol and any tips which I...
05 May 2016 2,625 0 View
I want to have a pair of primers for any gene or region 1.5-3.5 kb range so that I can use them as a positive control for yeast colony PCR (To test if my colony PCR is working) besides while...
02 February 2016 9,402 6 View
Does budding yeast SC has Spectraplakin Short Stop, an Actin–Microtubule cross-linker? If No then how does yeast MT-Actin interaction takes place or is there any other protein playing the same role?
01 January 2016 2,421 0 View
I have yeast genomic DNA (Faint band on the 0.9% gel). What PCR conditions and initial DNA(~ uL) I must take for getting amplification for the product size 2-3Kb?
10 October 2015 8,450 2 View
Isolated yeast genomic DNA (two samples), run on the 0.9%TAE agarose gel. Now could see both samples smear but different migration rate? Is it because of some left over ETOH if happened so while...
10 October 2015 7,325 3 View
