29 January 2016 3 3K Report

Is MX-1 breast cancer cell line P53 wild type or mutant?

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XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints. in K alpha2 value..can anyone help?

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints when entering K alpha value. Because of that I am not able to find the peaks...

11 June 2024 4,018 2 View

Crystal violet or hematoxylin is preferred for the staining of the Transwell membrane?

Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?

04 June 2024 2,766 0 View

Will a longer incubation of THP1-derived M2 macrophages on the assay plate have an impact on phagocytosis?

I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...

10 January 2024 538 1 View

How do we plot gain versus frequency plot in CST tool while designing an antenna?

I think there is no direct option to plot gain versus frequency in CST. kindly give solution

09 October 2023 8,738 3 View

What is the effect of DMSO in calcium assays?

What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...

28 September 2023 7,446 2 View

What is the optimized protocol for the Fluo 4 NW calcium assay in THP1-differentiated macrophages?

I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks

13 July 2023 4,985 0 View

What are the ideal mouse prostate cancer cell line to use in C57BL/6 mice?

I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.

13 June 2023 7,991 1 View

Any protocol or kit to measure intracellular calcium levels using a fluorescence microplate reader?

I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.

26 April 2023 2,036 2 View

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