Crystal violet or hematoxylin is preferred for the staining of the Transwell membrane?

Vahinipriya Manoharan @Vahinipriya-Manoharan

05 June 2024 0 3K Report

Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Vahinipriya Manoharan's questions See All

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,018 3 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints. in K alpha2 value..can anyone help?

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints when entering K alpha value. Because of that I am not able to find the peaks...

11 June 2024 4,017 2 View

Will a longer incubation of THP1-derived M2 macrophages on the assay plate have an impact on phagocytosis?

I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...

10 January 2024 538 1 View

How do we plot gain versus frequency plot in CST tool while designing an antenna?

I think there is no direct option to plot gain versus frequency in CST. kindly give solution

09 October 2023 8,738 3 View

What is the effect of DMSO in calcium assays?

What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...

28 September 2023 7,445 2 View

What is the optimized protocol for the Fluo 4 NW calcium assay in THP1-differentiated macrophages?

I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks

13 July 2023 4,985 0 View

What are the ideal mouse prostate cancer cell line to use in C57BL/6 mice?

I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.

13 June 2023 7,991 1 View

Any protocol or kit to measure intracellular calcium levels using a fluorescence microplate reader?

I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.

26 April 2023 2,035 2 View

Any recommendations for phagocytotic assay kits for THP1-derived macrophages?

I am planning to do some phagocytic assays on THP1-derived macrophages and am looking for a suitable phagocytic assay kit.

24 April 2023 285 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 725 3 View

Why Powder XRD is performed after cruhsing a crystal if Single Crystal XRD analysis of the same crystal has been performed already ?

What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?

30 July 2024 6,259 4 View

How to know the b axis of a single crystal?

How to apply magnetic field parallel to b axis of any crystal

29 July 2024 4,082 2 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,747 0 View

Is it possible to run the AIMD within a system using virtual crystal approximation (VCA)?

I want to study the thermal properties of a mixed system which is constructed by virtual crystal approximation in VASP. When I try to run the ab initio Molecular Dynamics of this system in VASP, I...

19 July 2024 6,568 3 View

Cresyl violet Nissl Staining ?

Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...

18 July 2024 2,420 0 View

What is the angular difference between two sets of Euler Angles?

Let's say I rotate a hexagonal crystal using Euler angles (φ1, φ2, φ3); and rotate another similar crystal using Euler angles (ψ1, ψ2, ψ3). What would be the angular difference between the final...

17 July 2024 5,582 1 View

How we can make this(as in figure) type of crystal structure (any software)?

I want to make this type of structure. Can anybody recommend the application for this?

15 July 2024 5,864 2 View

How to use stress and displacement results from 1st stationary simulation as initial conditions for 2nd eigenfrequency simulation in COMSOL?

Dear All, I am trying to define the dispersion curves of a periodic unit cell in COMSOL Multiphysics with prescribed displacements. I am using two separate studies: - Study 1: Stationary, with...

13 July 2024 7,855 1 View

How long takes to a batholith to crystallize?

How long would it take for a 'normal' granitic batholith to crystallize? Are there any calculations on it?

12 July 2024 7,436 0 View