Vahinipriya Manoharan

15 Questions 8 Answers 0 Followers

Questions related from Vahinipriya Manoharan

Crystal violet or hematoxylin is preferred for the staining of the Transwell membrane?

Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?

05 June 2024 2,708 0 View

Will a longer incubation of THP1-derived M2 macrophages on the assay plate have an impact on phagocytosis?

I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...

11 January 2024 502 1 View

What is the effect of DMSO in calcium assays?

What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...

29 September 2023 7,409 2 View

What is the optimized protocol for the Fluo 4 NW calcium assay in THP1-differentiated macrophages?

I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks

14 July 2023 4,949 0 View

What are the ideal mouse prostate cancer cell line to use in C57BL/6 mice?

I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.

14 June 2023 7,966 1 View

Any protocol or kit to measure intracellular calcium levels using a fluorescence microplate reader?

I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.

27 April 2023 2,016 2 View

Any recommendations for phagocytotic assay kits for THP1-derived macrophages?

I am planning to do some phagocytic assays on THP1-derived macrophages and am looking for a suitable phagocytic assay kit.

25 April 2023 265 2 View

In THP1 cells, how is a gene knocked out using siRNA?

Any optimized protocol for gene knockdown in THP1 cells using siRNA?

09 February 2023 6,541 0 View

Any colorimetric phagocytosis assays?

Please suggest any colorimetric based phagocytosis assays for THP1 differentiated macrophages

20 December 2022 6,596 3 View

Assay for THP1 differentiated macrophages?

I have the M1 and M2a subtypes of macrophages that I differentiated and polarized from THP-1 monocytes. I wanted to know the most suitable phagocytosis assays (preferably colorimetric method),...

05 December 2022 2,957 0 View

What genes are good qPCR markers for macrophage differentiation?

I am differentiating THP1 cells using PMA. Which are the good markers for determining human macrophage differentiation using qPCR?

30 June 2022 1,971 2 View

Can we do RT PCR with basal p53 expression?

I have extracted RNA from tumor tissue sample and wondering whether the basal level of p53 expression enough to do RT PCR amplification using p53 transcript specific primers? Thank you

21 September 2018 3,749 3 View

P53 mutation status of MX-1 breast cancer cell line?

Is MX-1 breast cancer cell line P53 wild type or mutant?

29 January 2016 2,507 3 View

Which will be the most suitable medium to culture MX-1 breast cancer cell line?

We cultured MX-1 in RPMI medium and the cells died. We are trying now with DMEM medium and still the growth is really slow. it is the 5th day and still it did not reach even 50% confluent. Is...

25 November 2015 8,463 2 View

Can we perform cell migration assays with THP1 differentiated and polarized cells?

I wanted to check the effect of my drug on the migration of M1 and M2 stages of THP1 cells. How can I do that? Once they are polarized to M1 and M2 stages, if I trypsinize them will they still...

01 January 1970 5,254 0 View