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Questions related from Vahinipriya Manoharan
I am performing a phagocytosis assay on mouse peritoneal cavity cells and detecting them using flow cytometry. Since the cells remain on the culture plate for 5 hours during the assay, they become...
22 July 2025 6,890 3 View
I am planning to assess the phagocytic activity of WT and gene-specific knockout macrophages using peritoneal cavity cells and flow cytometry. However, I’m wondering whether it is necessary to let...
20 June 2025 639 5 View
I am using 20 ng/mL PMA to differentiate U937 cells into M0 macrophages and then polarize them to M2 macrophages using IL-4 and IL-13. I wanted to collect these cells to perform a phagocytosis...
04 September 2024 2,341 4 View
I am finding it hard to trypsinize the M2-polarized U937 cells for functional assays, even when using 5 ng/ml PMA for differentiation. Any suggestions on how to lift the cells for seeding? Will...
21 August 2024 9,878 0 View
Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?
05 June 2024 2,818 0 View
I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...
11 January 2024 594 1 View
What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...
29 September 2023 7,486 2 View
I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks
14 July 2023 5,026 0 View
I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.
14 June 2023 8,051 1 View
I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.
27 April 2023 2,080 2 View
I am planning to do some phagocytic assays on THP1-derived macrophages and am looking for a suitable phagocytic assay kit.
25 April 2023 331 2 View
Any optimized protocol for gene knockdown in THP1 cells using siRNA?
09 February 2023 6,608 0 View
Please suggest any colorimetric based phagocytosis assays for THP1 differentiated macrophages
20 December 2022 6,645 3 View
I have the M1 and M2a subtypes of macrophages that I differentiated and polarized from THP-1 monocytes. I wanted to know the most suitable phagocytosis assays (preferably colorimetric method),...
05 December 2022 2,994 0 View
I am differentiating THP1 cells using PMA. Which are the good markers for determining human macrophage differentiation using qPCR?
30 June 2022 2,017 2 View
I have extracted RNA from tumor tissue sample and wondering whether the basal level of p53 expression enough to do RT PCR amplification using p53 transcript specific primers? Thank you
21 September 2018 3,822 3 View
Is MX-1 breast cancer cell line P53 wild type or mutant?
29 January 2016 2,581 3 View
We cultured MX-1 in RPMI medium and the cells died. We are trying now with DMEM medium and still the growth is really slow. it is the 5th day and still it did not reach even 50% confluent. Is...
25 November 2015 8,550 2 View
I am planning to analyse TP53 mutation using FASAY. I have got the P3/P4 primers (which has phosphorothioate linkage) as per in the published paper. I was trying to optimize the P3/P4 primers by...
14 September 2015 2,605 3 View
I wanted to check the effect of my drug on the migration of M1 and M2 stages of THP1 cells. How can I do that? Once they are polarized to M1 and M2 stages, if I trypsinize them will they still...
01 January 1970 5,306 0 View
