Normal endometrium explants culture.

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Scanning/analyzing zooplankton samples using a generic scanner?

Hello fellow scientists, Does anybody use a generic scanner to scan zooplankton samples? If so, what do you guys use in terms of software for segmentation of the original images into vignetes...

02 November 2023 8,565 0 View

We can found choaotic behavior in Biological Models Type SEIR, SEAIR, etc?

Dears, I am interested in chaotic behavior in classical biological models to spread any disease. To the best of my knowledge, I do not be able to find references that analyzed possible chaotic...

10 March 2023 7,281 5 View

Can anyone help me with Humberstone stain method for the simultaneous demonstration of Gram-positive and Gram-negative microorganisms in tissues?

Can anyone help me with Humberstone stain method for the simultaneous demonstration of Gram-positive and Gram-negative micro-organisms in paraffin sections?

21 December 2022 7,787 5 View

How to improve immunohistochemical staining in testis fixed with 4% PFA + 1.5% glutaraldehyde?

I am using antigen retrieval in citric acid (pH 6.0), followed by peroxidase blockade in H2O2+methanol and then serum blocking.

05 November 2021 5,687 2 View

Safe ambulation with femoral catheter?

how safe is it for routine ambulation if a patient has a femoral central line.? when would be safe enough to ambulate a pt. with a femoral central line

04 March 2021 7,006 3 View

Why my Chitosan (Medium Molecular Weight) sample is not soluble in HCl 2M but soluble in 2% Acetic acid?

I don't understand why my Chitosan sample (Sigma Aldrich Medium Molecular weight) is not soluble in HCl 2M solution (pH 1) but soluble in 2% Acetic acid solution (pH 4). For a hydrolisis protocol...

24 January 2021 2,096 3 View

How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 5,036 3 View

Why does my Phospho-4E-BP1 (Thr37/46) Antibody bind between 100-150kDa and not 15-20kDa?

Hi everyone. So I have tried to optimize my phospho-antibodies for western Blotting, however after several attempts the one antibody, Phospho-4E-BP1, keeps binding to the wrong protein. The...

19 October 2020 3,947 2 View

Are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

12 October 2020 9,102 1 View

Covalent coupling IgG to HPR using HPR glycan and IgG primary amine?

I am looking for detailed and simple protocol to couple IgG with HPR using HPR glycans and IgG amine using periodate reaction. Best if no dyalisis is required. I plann to use antibodies attached...

07 August 2020 9,499 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View