How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 4,865 3 View

Are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

12 October 2020 8,924 1 View

Simulation Parameters for p-type and n-type layers of a typical silicon soalr cell?

Hi, I would like to simulate a typical polycristalline silicon cell with p-type and n-type layers using a drift diffusion model. I have found a lot of references for intrinsic silicon parameter...

03 February 2020 3,203 6 View

Intrinsic tryptophan fluorescence of protein measured FI using a fluorescence microplater reader based on filter or monochromator?

I was wondering if microplate reader recording filter based flourescence emission spectrum are good to measure protein protein Intrinsic tryptophan fluorescence? How does it compare with...

13 January 2020 6,354 2 View

Can I use a monthly average solar spectrum to represent the spectrum of the month?

I would like to calculate the monthly average of the representative solar spectrum for each month based on minute spectral irradiance measurements. Can I consider this hypothesis? Has anyone made...

02 October 2019 7,380 4 View

Method for Identification of Bacterial Natural products by HPLC and LC-MS-MS using databases?

I would be grateful if you could please indicate public libraries used for the identification of natural products produced by bacterial using LC/MS/MS data and/or HPLC DAD analysis

16 September 2019 7,077 4 View

When you recommend to cut the heart in a murine models of heart failure?

Good evening everybody, I am working on a model of acute heart failure and I am planning to analyse heat shock proteins (western blot) in the right and the left ventricle. The mouse heart is very...

03 June 2019 7,528 5 View

Do you know successful Agriculture and Rural Credit Guarantee schemes?

Agriculture and Rural Credit Guarantee Schemes face additional challenges if compared with urban SME's Guarantee Schemes, especially related to climate, pricing and insurance issues. I would be...

17 March 2019 2,863 4 View

Can I substitute Qiagen Buffer QG (from dna extraction kit) for Buffer ERC (Minelute reaction cleanup)?

I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...

04 October 2017 8,150 3 View

Thoughts on performing PCR on purified, digested dna that has already been amplified (PCR) prior to digestion?

02 October 2017 3,916 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

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28 February 2021 9,936 2 View