How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 4,865 3 View

Simulation Parameters for p-type and n-type layers of a typical silicon soalr cell?

Hi, I would like to simulate a typical polycristalline silicon cell with p-type and n-type layers using a drift diffusion model. I have found a lot of references for intrinsic silicon parameter...

03 February 2020 3,203 6 View

Intrinsic tryptophan fluorescence of protein measured FI using a fluorescence microplater reader based on filter or monochromator?

I was wondering if microplate reader recording filter based flourescence emission spectrum are good to measure protein protein Intrinsic tryptophan fluorescence? How does it compare with...

13 January 2020 6,354 2 View

Can I use a monthly average solar spectrum to represent the spectrum of the month?

I would like to calculate the monthly average of the representative solar spectrum for each month based on minute spectral irradiance measurements. Can I consider this hypothesis? Has anyone made...

02 October 2019 7,380 4 View

Method for Identification of Bacterial Natural products by HPLC and LC-MS-MS using databases?

I would be grateful if you could please indicate public libraries used for the identification of natural products produced by bacterial using LC/MS/MS data and/or HPLC DAD analysis

16 September 2019 7,077 4 View

When you recommend to cut the heart in a murine models of heart failure?

Good evening everybody, I am working on a model of acute heart failure and I am planning to analyse heat shock proteins (western blot) in the right and the left ventricle. The mouse heart is very...

03 June 2019 7,528 5 View

Do you know successful Agriculture and Rural Credit Guarantee schemes?

Agriculture and Rural Credit Guarantee Schemes face additional challenges if compared with urban SME's Guarantee Schemes, especially related to climate, pricing and insurance issues. I would be...

17 March 2019 2,863 4 View

Can I substitute Qiagen Buffer QG (from dna extraction kit) for Buffer ERC (Minelute reaction cleanup)?

I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...

04 October 2017 8,150 3 View

Thoughts on performing PCR on purified, digested dna that has already been amplified (PCR) prior to digestion?

02 October 2017 3,916 3 View

Can I use DDT like RNAse inhibitor in a RT reaction?

Hi everybody , I need make cDNA from a low concentration of ARN ( lees than 50 ng).I found some MMLV that make cdna from lows ARN concentrations,but all of these use RNA inhibitors and DTT like...

07 February 2017 528 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View