22 December 2022 5 8K Report

Can anyone help me with Humberstone stain method for the simultaneous demonstration of Gram-positive and Gram-negative micro-organisms in paraffin sections?

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Scanning/analyzing zooplankton samples using a generic scanner?

Hello fellow scientists, Does anybody use a generic scanner to scan zooplankton samples? If so, what do you guys use in terms of software for segmentation of the original images into vignetes...

02 November 2023 8,565 0 View

We can found choaotic behavior in Biological Models Type SEIR, SEAIR, etc?

Dears, I am interested in chaotic behavior in classical biological models to spread any disease. To the best of my knowledge, I do not be able to find references that analyzed possible chaotic...

10 March 2023 7,281 5 View

How to improve immunohistochemical staining in testis fixed with 4% PFA + 1.5% glutaraldehyde?

I am using antigen retrieval in citric acid (pH 6.0), followed by peroxidase blockade in H2O2+methanol and then serum blocking.

05 November 2021 5,687 2 View

Safe ambulation with femoral catheter?

how safe is it for routine ambulation if a patient has a femoral central line.? when would be safe enough to ambulate a pt. with a femoral central line

04 March 2021 7,006 3 View

Why my Chitosan (Medium Molecular Weight) sample is not soluble in HCl 2M but soluble in 2% Acetic acid?

I don't understand why my Chitosan sample (Sigma Aldrich Medium Molecular weight) is not soluble in HCl 2M solution (pH 1) but soluble in 2% Acetic acid solution (pH 4). For a hydrolisis protocol...

24 January 2021 2,096 3 View

How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 5,036 3 View

Why does my Phospho-4E-BP1 (Thr37/46) Antibody bind between 100-150kDa and not 15-20kDa?

Hi everyone. So I have tried to optimize my phospho-antibodies for western Blotting, however after several attempts the one antibody, Phospho-4E-BP1, keeps binding to the wrong protein. The...

19 October 2020 3,947 2 View

Are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

12 October 2020 9,102 1 View

Covalent coupling IgG to HPR using HPR glycan and IgG primary amine?

I am looking for detailed and simple protocol to couple IgG with HPR using HPR glycans and IgG amine using periodate reaction. Best if no dyalisis is required. I plann to use antibodies attached...

07 August 2020 9,499 0 View

Does anybody know the stability of SARS-CoV-2 N protein structure in response to heat (Tm) and Tween 80 0.1%?

Does anybody know the stability of SARS-CoV-2 N protein structure in response to heat (Tm) and Tween 80 0.1%? Or even heat and tween combined?

28 June 2020 2,902 4 View

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I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

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How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

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Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?

What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?

31 July 2024 3,965 0 View

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