10 February 2020 4 3K Report

I need to remove the coating of NiCrAlY from SLG and IN718 substrates. Do anyone can give me a solution?

More Dasari Mohan Chandra's questions See All
How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Why do these abnormal charge/discharge curves of coin cell batteries appear?

As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...

24 July 2024 6,930 1 View

How to troubleshoot the Milliplex Cytokine assay, when I see >OOR/<OOR in my data?

I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....

24 June 2024 9,717 0 View

How can formulate Phosphorus 52 and Potassium 34 in liquid ?

formulate Phosphorus 52 and Potassium 34 in liquid

03 June 2024 7,589 2 View

"Range chek error" problem in DSSAT Model?

I was previously using the DSSAT version 4.7 in my PC. Now, I have installed the DSSAT v4.8. Whenever I am trying to simulate the experient is shows "Range chek error". This problem exist even...

13 May 2024 5,894 0 View

How to calculate sensitivity, LoD and LoQ from a DPV plot of peak current against logarithm of concentration?

In some electrochemistry research papers, the result obtained from a differential pulse voltammetry (DPV) technique is presented in peak current vs. log(concentration) plot. in such cases, how to...

04 May 2024 9,605 2 View

I am trying to do X ray peak broadening analysis of nanosheets. How important it is to strip the K-alpha 2 for accurate results?

The stress-strain calculations, energy density GI index calculations are also being done.

02 May 2024 9,963 4 View

How have solitons been interpreted in the protein folding mechanism?

One and two soliton solutions have been obtained while considering the protein folding mechanism of Davydov solitons. What are the dynamics of these soliton solutions that can be obtained in...

25 April 2024 5,241 0 View

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

Has anyone designed guideRNA for multiple targets using polycistronic-tRNA-gRNA expression system?

I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...

29 March 2024 1,055 0 View

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

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Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

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How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

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Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

What procedure should I follow for current controlled PPy film electrochemical deposition on ITO?

For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...

06 August 2024 3,176 0 View

How can I measure the thickness of thin film by Uv-Vis?

How can I measure the thickness of thin film by Uv-Vis?

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Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of  absorption chromatography?

03 August 2024 7,035 3 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

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