One and two soliton solutions have been obtained while considering the protein folding mechanism of Davydov solitons. What are the dynamics of these soliton solutions that can be obtained in these studies?
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...
24 July 2024 6,930 1 View
I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....
24 June 2024 9,717 0 View
formulate Phosphorus 52 and Potassium 34 in liquid
03 June 2024 7,589 2 View
I was previously using the DSSAT version 4.7 in my PC. Now, I have installed the DSSAT v4.8. Whenever I am trying to simulate the experient is shows "Range chek error". This problem exist even...
13 May 2024 5,894 0 View
The stress-strain calculations, energy density GI index calculations are also being done.
02 May 2024 9,962 4 View
I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...
29 March 2024 1,055 0 View
The answer help young researchers get motivated
19 March 2024 3,203 1 View
Procedure for the preparation of 10% Boron liquid.
20 December 2023 9,621 2 View
As we are talking about artificial intelligence on every platform,. Most of the stakeholders try to undertake this new technology in the public health field. Let us share our experience about...
19 December 2023 2,341 8 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,175 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,987 3 View
How can I interpret the data gathered without solving?
03 August 2024 9,052 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,891 4 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,005 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 365 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,737 0 View