Has anyone designed guideRNA for multiple targets using polycistronic-tRNA-gRNA expression system?

30 March 2024 0 1K Report

I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA for proper expression and processing. Any suggestions in this regard would be grateful.

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