How can formulate Phosphorus 52 and Potassium 34 in liquid ?

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How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Why do these abnormal charge/discharge curves of coin cell batteries appear?

As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...

24 July 2024 6,930 1 View

How to troubleshoot the Milliplex Cytokine assay, when I see >OOR/<OOR in my data?

I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....

24 June 2024 9,717 0 View

"Range chek error" problem in DSSAT Model?

I was previously using the DSSAT version 4.7 in my PC. Now, I have installed the DSSAT v4.8. Whenever I am trying to simulate the experient is shows "Range chek error". This problem exist even...

13 May 2024 5,894 0 View

I am trying to do X ray peak broadening analysis of nanosheets. How important it is to strip the K-alpha 2 for accurate results?

The stress-strain calculations, energy density GI index calculations are also being done.

02 May 2024 9,962 4 View

How have solitons been interpreted in the protein folding mechanism?

One and two soliton solutions have been obtained while considering the protein folding mechanism of Davydov solitons. What are the dynamics of these soliton solutions that can be obtained in...

25 April 2024 5,240 0 View

Has anyone designed guideRNA for multiple targets using polycistronic-tRNA-gRNA expression system?

I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...

29 March 2024 1,054 0 View

In RG 80 Nobel Prize Winners are listed ,Can we know their area of research and institute of affiliation with country ?

The answer help young researchers get motivated

19 March 2024 3,203 1 View

How to make 10% Boron solution , which content 10% elemental Boron value ?

Procedure for the preparation of 10% Boron liquid.

20 December 2023 9,620 2 View

How can we start artificial intelligence in public health?

As we are talking about artificial intelligence on every platform,. Most of the stakeholders try to undertake this new technology in the public health field. Let us share our experience about...

19 December 2023 2,341 8 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 612 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,609 0 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,694 0 View

How to convert available phosphorus to total phosphorus?

I need to convert Available phosphorus into total phosphorus

18 July 2024 6,797 3 View

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 318 1 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,974 1 View

Asked a separate ways?

Dear all, I have a question regarding ways to separate ammonium nitrate and phosphorus pentoxide？

12 July 2024 3,164 2 View