I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and Tag experiment? I am following Steven Henikoff 2020 CUT & Tag protocol.
TAPS ([tris(hydroxymethyl)methylamino]propanesulfonic acid) pH range 7.7–9.1, pKa value of 8.44 (ionic strength I = 0, 25 °C)