Currently, there are many methods or algorithms to predict neoantigen in human samples. However, if we would like to predict neoantigen from murine cancer cell lines in C57BL6/J background, does anyone know any available publication or online tools?
pVacseq + NetMHCpan
Dear all, I am new to TEM that I would like to know if there's any dendritic cell specific organelle under TEM observation? Birbeck body/ granule seems to be specific only for skin...
01 February 2017 6,010 1 View
Dear All, We are trying to quantify dna content by Hoechst 33342 staining after seahorse experiment. One of our cell conditions consistently yield higher hoechst staining readouts that we...
10 November 2016 7,195 3 View
Hi, I have isolated some mouse splenocyte and collected the pellets after isolation. I added QIAZOL into the cell pellet, but I forgot to pipette it up-and-down before I put it into the -80...
10 November 2015 473 8 View
Does anyone know or have a breast cancer murine model to study recurrence? I have checked the literature, but did not find relavant references. I am not sure whether it was because there's no good...
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I am looking for murine ER positive cell line now. However, from ATCC website, Estrogen receptor status was not reported. While checking other publication, C4HD cell line was found. But, C4HD is...
03 April 2014 9,179 3 View
We are working on MCF7 tumor model on Balb/c AnN.Cg-Foxn1nu/CrlNarl nude mice now. Concerning the estrogen supplement, are there methods other than daily subcutaneous injection that work well? If...
31 December 2013 1,310 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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