Are there any Dendritic cell specific organelle under Transmission Electron Microscopy?

01 February 2017 5,857 1 View

Will lipid droplets interefere with Hoechst 33342 staining for DNA quantification?

10 November 2016 7,088 3 View

Cell pellet in trizol and subsequent RNA extraction?

Hi, I have isolated some mouse splenocyte and collected the pellets after isolation. I added QIAZOL into the cell pellet, but I forgot to pipette it up-and-down before I put it into the -80...

10 November 2015 279 8 View

Are there any murine models breast cancer recurrence ?

Does anyone know or have a breast cancer murine model to study recurrence? I have checked the literature, but did not find relavant references. I am not sure whether it was because there's no good...

06 July 2015 7,330 4 View

Can someone advise on murine estrogen receptor positive cell line?

I am looking for murine ER positive cell line now. However, from ATCC website, Estrogen receptor status was not reported. While checking other publication, C4HD cell line was found. But, C4HD is...

03 April 2014 9,071 3 View

What kind of estrogen supplement should I use for my MCF7 tumorigenicity in nude mice model?

We are working on MCF7 tumor model on Balb/c AnN.Cg-Foxn1nu/CrlNarl nude mice now. Concerning the estrogen supplement, are there methods other than daily subcutaneous injection that work well? If...

31 December 2013 1,141 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View