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Questions related from Ichun Chen
Currently, there are many methods or algorithms to predict neoantigen in human samples. However, if we would like to predict neoantigen from murine cancer cell lines in C57BL6/J background, does...
02 February 2018 3,470 1 View
Dear all, I am new to TEM that I would like to know if there's any dendritic cell specific organelle under TEM observation? Birbeck body/ granule seems to be specific only for skin...
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Dear All, We are trying to quantify dna content by Hoechst 33342 staining after seahorse experiment. One of our cell conditions consistently yield higher hoechst staining readouts that we...
11 November 2016 7,159 3 View
Hi, I have isolated some mouse splenocyte and collected the pellets after isolation. I added QIAZOL into the cell pellet, but I forgot to pipette it up-and-down before I put it into the -80...
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Does anyone know or have a breast cancer murine model to study recurrence? I have checked the literature, but did not find relavant references. I am not sure whether it was because there's no good...
07 July 2015 7,397 4 View
I am looking for murine ER positive cell line now. However, from ATCC website, Estrogen receptor status was not reported. While checking other publication, C4HD cell line was found. But, C4HD is...
04 April 2014 9,135 3 View
We are working on MCF7 tumor model on Balb/c AnN.Cg-Foxn1nu/CrlNarl nude mice now. Concerning the estrogen supplement, are there methods other than daily subcutaneous injection that work well? If...
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