Dear All,
We are trying to quantify dna content by Hoechst 33342 staining after seahorse experiment. One of our cell conditions consistently yield higher hoechst staining readouts that we are wondering if it is true or it is because of artifact caused by lipid droplets in the cell. Will lipid content in the cells interfere with Hoechst staining? Are there other suggested methods for DNA content quantification after seahorse experiment?
Thanks for your help
May I suggest the following article:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2976661/
Lipid Droplets generally do not interfere with Hoechst staining. We do Hoechst staining of zebrafish embryos...it stains the nucleus alone...u can see this article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3870878/
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining